2P216 Laser Raman spectroscopic detection of iron compounds in magnetotactic bacterium, Magnetospirillum magnetotacticum MS-1(Cell biological problems-adhesion, motility, cytoskeleton, signaling, and membrane,Poster Presentations)
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Nitrate reductase from the magnetotactic bacterium Magnetospirillum magnetotacticum MS-1: purification and sequence analyses.
We purified the nitrate reductase from the soluble fraction of Magnetospirillum magnetotacticum MS-1. The enzyme was composed of 86- and 17-kDa subunits and contained molybdenum, non-heme iron, and heme c. These properties are very similar to those of the periplasmic nitrate reductase found in Paracoccus pantotrophus. The M. magnetotacticum nap locus was clustered in seven open reading frames, ...
متن کاملIron reductase for magnetite synthesis in the magnetotactic bacterium Magnetospirillum magnetotacticum.
Ferric iron reductase was purified from magnetotactic bacterium Magnetospirillum (formerly Aquaspirillum) magnetotacticum (ATCC 31632) to an electrophoretically homogeneous state. The enzyme was loosely bound on the cytoplasmic face of the cytoplasmic membrane and was found more frequently in magnetic cells than in nonmagnetic cells. The molecular mass of the purified enzyme was calculated upon...
متن کاملEnzymatic Iron Oxidation and Reduction in Magnetite Synthesizing Magnetospirillum Magnetotacticum
We investigated the enzymatic reduction and oxidation of iron in M.magnetotacticum which synthesizes magnetite at room temperature. NADH-Fe(lI1) reductase with the molecular mass of 36kDa was purified from the bacterium. The enzyme was located in cytoplasm and utilized NADH and NADPH in the presence of FMN as reductant and showed maximum activity at pH 7.0. The Km for NADH and NADPH were about ...
متن کاملGenome Sequence of Magnetospirillum magnetotacticum Strain MS-1
Here, we report the genome sequence of Magnetospirillum magnetotacticum strain MS-1, which consists of of 36 contigs and 4,136 protein-coding genes.
متن کاملEffect of Polyethylene Glycol on the Formation of Magnetic Nanoparticles Synthesized by Magnetospirillum magnetotacticum MS-1
Magnetotactic bacteria (MTB) synthesize intracellular magnetic nanocrystals called magnetosomes, which are composed of either magnetite (Fe3O4) or greigite (Fe3S4) and covered with lipid membranes. The production of magnetosomes is achieved by the biomineralization process with strict control over the formation of magnetosome membrane vesicles, uptake and transport of iron ions, and synthesis o...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2007
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.47.s167_1